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anti vdac  (Proteintech)


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    Structured Review

    Proteintech anti vdac
    Anti Vdac, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 185 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+vdac/VDAC1%2F2+Antibody/10__1038_slash_s41467___026___78424___w-288-38-39
    Average 95 stars, based on 185 article reviews
    anti vdac - by Bioz Stars, 2026-11
    95/100 stars

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    Related Articles

    Incubation:

    Article Title: Critical role of translocator protein (TSPO) in neuronal mitochondrial dysfunction and mental stress-exacerbated ischemic injury following stroke
    Article Snippet: .. Primary antibodies were added and incubated at 4 °C overnight: anti–VDAC (1:500, 66345-1-Ig, Proteintech), anti-ACBD3 (1:500, 14096-1-AP, Proteintech), anti–TSPO (66345-1-Ig, Proteintech), anti-pan phospho-serine/threonine (1:500, AP0893, abclonal), and IgG antibody (1:1000, 2729, Cell Signaling Technology). .. Protein G magnetic beads were added, and magnetic separation was performed using a Magnetic Separation Rack (7017, CST).

    Article Title: Critical role of translocator protein (TSPO) in neuronal mitochondrial dysfunction and mental stress-exacerbated ischemic injury following stroke.
    Article Snippet: .. Primary antibodies were added and incubated at 4 °C overnight: anti–VDAC (1:500, 66345-1-Ig, Proteintech), anti-ACBD3 (1:500, 14096-1-AP, Proteintech), anti–TSPO (66345-1-Ig, Proteintech), anti-pan phospho-serine/ threonine (1:500, AP0893, abclonal), and IgG antibody (1:1000, 2729, Cell Signaling Technology). .. Protein G magnetic beads were added, and magnetic separation was performed using a Magnetic Separation Rack (7017, CST).

    Article Title: ACAT1/SOAT1 governs mitochondrial dynamics and thermogenesis via the mTOR-MFN2-USP2 axis in brown adipocytes
    Article Snippet: Proteins were separated by SDS-PAGE and transferred electrophoretically to a PVDF membrane (Sigma-Aldrich, 24937-79-9). .. Membranes were incubated with primary antibodies overnight at 4 °C: anti-ACAT1 (Purified by our lab), anti-HSP90 (ProteinTech, 60318-1-Ig), anti-Beta Actin (ProteinTech, 66009-1-Ig), antiOXPHOS (Abcam, ab110413), anti-UCP1 (Abcam, ab10983), antiCalnexin (ProteinTech, 10427-2-AP), anti-IP3R (ProteinTech, 19962-1- AP), anti-FACL4 (Abcam, ab155282), anti-VDAC (ProteinTech, 10866-1- AP), anti-Tubulin (ProteinTech, 66031-1-Ig), anti-MFN2 (ProteinTech, 12186-1-AP), anti-MFN1 (ProteinTech, 66776-1-Ig), anti-OPA1 (ProteinTech, 27733-1-AP), anti-DRP1 (ProteinTech, 12957-1-AP), antipSer616-DRP1 (Cell Signaling, 3455), anti-MFF (ProteinTech, 17090-1- AP), anti-Flag (Sigma, F1804), anti-P4D1 (Santa Cruz, sc-8017), anti-HA (ProteinTech, 51064-1-AP), anti-mTOR (ProteinTech, 66888-1-Ig), antipSer2448-mTOR (Cell signaling, 5536), anti-pThr389-p70S6Kinase (Cell signaling, 9234), anti-USP2 (ProteinTech, 10392-1-AP) and antiGNPAT (ProteinTech, 14931-1-AP). .. After incubation with primary antibodies, membranes were washed with TBST and incubated with secondary antibodies conjugated to horseradish peroxidase (HRP; Epizyme, LF101 and LF102) at room temperature for 1 hour.

    other:

    Article Title: Harnessing tissue-derived mitochondria-rich extracellular vesicles (Ti-mitoEVs) to boost mitochondrial biogenesis for regenerative medicine
    Article Snippet: EV protein was extracted using radioimmunoprecipitation assay buffer (Beyotime) containing protease inhibitors (Calbiochem, San Diego, CA, USA).

    Purification:

    Article Title: ACAT1/SOAT1 governs mitochondrial dynamics and thermogenesis via the mTOR-MFN2-USP2 axis in brown adipocytes
    Article Snippet: Proteins were separated by SDS-PAGE and transferred electrophoretically to a PVDF membrane (Sigma-Aldrich, 24937-79-9). .. Membranes were incubated with primary antibodies overnight at 4 °C: anti-ACAT1 (Purified by our lab), anti-HSP90 (ProteinTech, 60318-1-Ig), anti-Beta Actin (ProteinTech, 66009-1-Ig), antiOXPHOS (Abcam, ab110413), anti-UCP1 (Abcam, ab10983), antiCalnexin (ProteinTech, 10427-2-AP), anti-IP3R (ProteinTech, 19962-1- AP), anti-FACL4 (Abcam, ab155282), anti-VDAC (ProteinTech, 10866-1- AP), anti-Tubulin (ProteinTech, 66031-1-Ig), anti-MFN2 (ProteinTech, 12186-1-AP), anti-MFN1 (ProteinTech, 66776-1-Ig), anti-OPA1 (ProteinTech, 27733-1-AP), anti-DRP1 (ProteinTech, 12957-1-AP), antipSer616-DRP1 (Cell Signaling, 3455), anti-MFF (ProteinTech, 17090-1- AP), anti-Flag (Sigma, F1804), anti-P4D1 (Santa Cruz, sc-8017), anti-HA (ProteinTech, 51064-1-AP), anti-mTOR (ProteinTech, 66888-1-Ig), antipSer2448-mTOR (Cell signaling, 5536), anti-pThr389-p70S6Kinase (Cell signaling, 9234), anti-USP2 (ProteinTech, 10392-1-AP) and antiGNPAT (ProteinTech, 14931-1-AP). .. After incubation with primary antibodies, membranes were washed with TBST and incubated with secondary antibodies conjugated to horseradish peroxidase (HRP; Epizyme, LF101 and LF102) at room temperature for 1 hour.

    Cell-Signaling:

    Article Title: ACAT1/SOAT1 governs mitochondrial dynamics and thermogenesis via the mTOR-MFN2-USP2 axis in brown adipocytes
    Article Snippet: Proteins were separated by SDS-PAGE and transferred electrophoretically to a PVDF membrane (Sigma-Aldrich, 24937-79-9). .. Membranes were incubated with primary antibodies overnight at 4 °C: anti-ACAT1 (Purified by our lab), anti-HSP90 (ProteinTech, 60318-1-Ig), anti-Beta Actin (ProteinTech, 66009-1-Ig), antiOXPHOS (Abcam, ab110413), anti-UCP1 (Abcam, ab10983), antiCalnexin (ProteinTech, 10427-2-AP), anti-IP3R (ProteinTech, 19962-1- AP), anti-FACL4 (Abcam, ab155282), anti-VDAC (ProteinTech, 10866-1- AP), anti-Tubulin (ProteinTech, 66031-1-Ig), anti-MFN2 (ProteinTech, 12186-1-AP), anti-MFN1 (ProteinTech, 66776-1-Ig), anti-OPA1 (ProteinTech, 27733-1-AP), anti-DRP1 (ProteinTech, 12957-1-AP), antipSer616-DRP1 (Cell Signaling, 3455), anti-MFF (ProteinTech, 17090-1- AP), anti-Flag (Sigma, F1804), anti-P4D1 (Santa Cruz, sc-8017), anti-HA (ProteinTech, 51064-1-AP), anti-mTOR (ProteinTech, 66888-1-Ig), antipSer2448-mTOR (Cell signaling, 5536), anti-pThr389-p70S6Kinase (Cell signaling, 9234), anti-USP2 (ProteinTech, 10392-1-AP) and antiGNPAT (ProteinTech, 14931-1-AP). .. After incubation with primary antibodies, membranes were washed with TBST and incubated with secondary antibodies conjugated to horseradish peroxidase (HRP; Epizyme, LF101 and LF102) at room temperature for 1 hour.



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    A. Swelling of BAT mitochondria from WT and Ucp1 -/- mice upon 3 weeks of cold exposure. B. Swelling of mitochondria from liver of WT mice kept at RT for comparison. C. Immunoblots of proteins implicated in <t>ERMCs:</t> <t>GRP75,</t> IP3R, <t>VDAC,</t> and cyclophilin D (CyD). D. Quantified protein expression. Calnexin, TOM20 and Na/K ATPase were used for normalization per ER biomass, mitochondrial biomass, and total extract, respectively. E. Representative image of PLA for IP3R–VDAC in BAT of WT and Ucp1 -/- mice exposed to 6°C for 3 weeks. F. Quantification of the contact sites from E . n = 4-6 mice. Data shown as mean ± sem. Unpaired t-test.
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    Image Search Results


    A. Swelling of BAT mitochondria from WT and Ucp1 -/- mice upon 3 weeks of cold exposure. B. Swelling of mitochondria from liver of WT mice kept at RT for comparison. C. Immunoblots of proteins implicated in ERMCs: GRP75, IP3R, VDAC, and cyclophilin D (CyD). D. Quantified protein expression. Calnexin, TOM20 and Na/K ATPase were used for normalization per ER biomass, mitochondrial biomass, and total extract, respectively. E. Representative image of PLA for IP3R–VDAC in BAT of WT and Ucp1 -/- mice exposed to 6°C for 3 weeks. F. Quantification of the contact sites from E . n = 4-6 mice. Data shown as mean ± sem. Unpaired t-test.

    Journal: bioRxiv

    Article Title: Chronic cold exposure induces plasticity of mitochondrial calcium uptake in beige and brown fat of UCP1-deficient mice

    doi: 10.64898/2026.03.16.712209

    Figure Lengend Snippet: A. Swelling of BAT mitochondria from WT and Ucp1 -/- mice upon 3 weeks of cold exposure. B. Swelling of mitochondria from liver of WT mice kept at RT for comparison. C. Immunoblots of proteins implicated in ERMCs: GRP75, IP3R, VDAC, and cyclophilin D (CyD). D. Quantified protein expression. Calnexin, TOM20 and Na/K ATPase were used for normalization per ER biomass, mitochondrial biomass, and total extract, respectively. E. Representative image of PLA for IP3R–VDAC in BAT of WT and Ucp1 -/- mice exposed to 6°C for 3 weeks. F. Quantification of the contact sites from E . n = 4-6 mice. Data shown as mean ± sem. Unpaired t-test.

    Article Snippet: Lysates were resolved by sodium dodecyl sulfate (SDS)–polyacrylamide gel electrophoresis (PAGE), transferred to a polyvinylidene difluoride (PVDF) membrane (Millipore), and probed with antibodies against Na + /K + -ATPase (Abcam, ab76020, GR3237646-13, 1/10,000), TOMM20 (Sigma Prestige, HPA011562, 1/500), MCU (Cell Signaling Technology, 14997, 1/100), UCP1 (Abcam, ab10983, 1/1000), NCLX (Proteintech, 21430-1-AP, 1/500), TMBIM5 (Proteintech, 16296-1-AP, 1/500), TMEM65 (Invitrogen, PA5-112762, 1/500), GRP75 (Abcam, ab53098, 1/500), Calnexin (BD Transduction Laboratories, 610523, 1/1000), VDAC (Cell Signaling Technology, 4661, 1/2000), CyD (Proteintech, 18466-1-AP, 1/1000), OXPHOS (Abcam, ab110413, 1/500), Vinculin (1:10000; V9131; Sigma-Aldrich), NDUFB6 (1:5000; #6510832 Thermo Fisher Scientific), mtCOI (1:10000; #10444035; Thermo Fisher Scientific), ATP5A (1:1,000; #439800; Thermo Fisher Scientific), SDHA (1:1,000, #459200, Thermo Fisher Scientific), SDHB (1:1,000, #ab14714, Abcam), UQCRC2 (1:1,000, #14742-1-AP; Proteintech), ATPIF1 (1:1,000, #13268S; Cell Signaling Technology), TOMM20 Alexa Fluor 488 (1:1,000; #ab205486; Abcam), and cytochrome c (1:5,000, #ab110325, Abcam).

    Techniques: Comparison, Western Blot, Expressing